Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR.
نویسندگان
چکیده
A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 71 4 شماره
صفحات -
تاریخ انتشار 2005